AmScope ME580 Series - Operation; Setting up; Focusing; Centering Bulb Position

14
ME580 Series
User’s Manual
Operaon
Seng Up
1. Place the specimen to be studied on the stage (if opaque) or glass slide (if translucent and using a -2L mod-
el). Place it on the stage, holding it snugly in place with the metal slide holders (clips) of the stage. You may
need to place the stage plate on the stage if you are using a -2L model that has a hole in the stage.
2. Using the mechanical stage controls, center the specimen, lining it up with the light and the objecve lens.
3. To adjust the illuminaon (boom light only for -2L models), slowly turn the dimmer on the right of the base
of the microscope unl the desired intensity of light is achieved. The upper illuminator brightness is not adjust-
ed by a dimmer, but is adjustd by the aperture and eld adjustments on the light path itself.
Focusing
1. Turn the nosepiece to choose an objecve. It is easiest to use the lowest magnicaon rst (4x objecve,
usually the shortest) to locate and focus on the specimen. As you move higher in magnicaon by switching
lenses, you may need to refocus the image a lile each me.
2. Being focusing by rst adjusng the distance between each eyepiece to t the distance between your eyes
by sliding them towards or away from each other as needed. Then, look with one eye through the right eye-
piece. Posion the diopter so that the eyepiece is as close to the microscope as possible. Use the coarse focus-
ing knob to adjust unl the sample comes into clear focus. Use the diopter on the other eyepiece to adjust
unl the images collimate (align so you see a single matching image through both eyes).
3. Once the image is mostly clear in your eld of view, use the ne focusing knob to tune it for best results.
4. Once you have focused on your sample, you may adjust the slide to recenter on the area you wish to focus
on, then move to the next highest objecve. Repeat steps 2 through 4 unl the magnicaon you wish to view
at is focused.
Centering Bulb Posion
1. The upper illuminator best illuminates the sample when the bulb is posioned in the center of the light
path. This is done using adjustment screws on the bulb housing module.
2. To center the bulb, remove an objecve from the turret, and set that hole as the opening in the path of light
on the microscope’s nosepiece. Place a sheet of paper on the stage.
3. While looking at the circle of light on the paper, adjust the bulb’s X and Y posion adjustment screws unl
the brightest point visible is centered on the sheet of paper.