AmScope ME580 Series - Upper Illuminator Setup & Use

15
ME580 Series
User’s Manual
Operaon
Upper Illuminator Setup & Use
1. The upper illuminaon method is used when the sample being viewed is an opaque sample, meaning, light
does not pass through it. Upper illuminaon comes through the objecves, bounces o of the opaque sample,
then re-enters the objecve, creang an image visible to the user. This is called a “reected” illuminator style,
as it reects o of the sample to form an image, rather than through a translucent sample like a tradional
compound microscope.
2. To turn the upper illuminator on, the switch on the right side of the microscope body must be ipped, and
the knob adjusted for intensity unl the light is visible or the desired brightness is achieved when looking
through the eyepieces.
3. For the best image quality when using the upper illuminator, the eld diaphragm must be adjusted to be just
larger than the current eld of view when looking in the microscope, and centered. The angled screws on the
illuminaon path are used to center the iris diaphragm, while the sliding bar is used to adjust iris size.
4. Begin by shrinking the iris to its smallest seng, then adjusng the screws to get the small iris in the center
of the eld of view, by looking into the microscope. Once centered, open the iris and check to see if the iris is
completely inside the eld of view, or if it is o to one parcular side. If o, adjust as necessary unl centered.
5. Once centered, open the iris just beyond the edge of the eld of view as seen in the microscope. This is the
posion for the best possible image when focused.
6. The aperture diaphragm must also be adjusted, as this helps determine the numerical aperture of the
system. Matching this to the objecve in use provides the best image, and is done by viewing the sample and
adjusng the aperture diaphgram slider bar for opmal contrast and clarity while looking in the microscope.