BD FACSCelesta Flow Cytometer User’s Guide120
Air bubble or debris in the
flow cell
Prime the fluidics system. See Priming
the fluidics (page 40).
No sample in the tube Verify that sample remains in the tube
and if necessary, add sample to the
tube or install a new sample tube.
Sample is not mixed properly Mix the sample to suspend the cells.
Waste tank is full Empty the waste tank.
PMT voltages set too low or
too high for display
parameter
Adjust the PMT voltages.
Too few events are displayed Increase the number of events to
display.
Sample injection tube is
clogged
Remove the sample tube to allow
backflushing.
If the event rate is still erratic, clean
the sample injection tube. See Cleaning
the fluidics (page 47).
Bal seal is worn Replace the Bal seal. See Changing the
Bal seal (page 56).
Instrument is not warmed up Wait 30 minutes for the instrument to
warm up.
Laser is not functioning Verify the malfunction by changing the
threshold to an alternative laser while
running the appropriate sample. If
unsuccessful, contact BD Biosciences.
Tube is cracked or misshapen Replace the sample tube.
Waste tank is pressurized. Disconnect
the waste container for at least
30 seconds to allow the pressure to
dissipate.
Possible causes Recommended solutions
To Next Page
Loading...
