BD FACSCelesta Flow Cytometer - Page 24

BD FACSCelesta Flow Cytometer User’s Guide24
Fluidic alarms and
the Mode button
The fluidic alarms are triggered by the waste and sheath fluid levels
in the tanks. The alarms sound when the waste tank is nearly
100% full and the sheath tank is empty. The fluidic alarms and
system status will also show warnings when you start up the
cytometer until the tank pressure reaches the correct level.
To silence the alarm, press the MODE button, then press the Down
button. The MODE button flashes to indicate the cytometer is in
silent mode. Repeat this sequence to turn off silent mode.
Note: Turning the cytometer to silent mode may result in overflow
of waste and you may run out of sheath.
Note: When the cytometer is in HTS mode, both visual and
audible alarms will be deactivated.
Line Definition
1
Waste level. Shows range from E (empty) to F (full). The
display line increases from left to right in sequences of
20%. System status turns yellow at 80%, and red at 100%
full.
2
Sheath level. Shows range from E to F. The display line
decreases from right to left in sequences of 20% from full
level. System status turns yellow at 20%, and red at 0%.
3
Fine Adj. Shows the current setting of fine adjust. Fine
adjust can be set in increments of 10 from 0 to 500. The
normal set point is 250. The last value persists, even after
cytometer shutdown.
4
HTS mode. Shows that the cytometer is in HTS mode. To
enter this mode, press and hold down the MODE button
for more than 3 seconds. Status information is not
displayed and system status turns green. To return to the
normal mode, press and hold the MODE button for more
than 3 seconds