Chapter 3: Cytometer setup 39
Biological
precautions
To prevent exposure to biohazardous agents:
• Put the cytometer in standby mode before disconnecting the
waste tank to avoid leakage of biohazardous waste.
• Always disconnect the waste container from the cytometer
before you empty it. Wait at least 30
seconds for pressure to
dissipate before you remove the waste cap or sensor.
• Expose waste container contents to bleach (10% of total
volume) for 30
minutes before disposal.
• Do not wet the waste tank cap. If wet, the filter in the cap will
cause the tank to malfunction. To keep the cap dry, place it on
the bench label side up when it is not on the tank.
Procedure To prepare the waste container:
1. Verify that the flow cytometer is in standby mode.
Press the STANDBY button on the control panel if necessary.
2. Disconnect the orange waste tubing from the waste container.
3. Disconnect the black alarm sensor line from the alarm sensor
socket.
Keep the lid and moisture trap on the waste container until
you are ready to empty it.
4. Remove the lid and moisture trap before emptying the waste
container. Keep the cap label side up at all times. Empty the
waste container.
5. Add approximately 1 L of bleach to the waste container and
close it. Reattach the moisture trap and lid.
Caution: Biohazard! Contact with biological specimens
and materials can transmit potentially fatal disease.
Caution! The waste container is heavy when full.
When emptying it, use good body mechanics to
prevent injury.
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