Chapter 7: Troubleshooting 103
Sample tube not
fitting on SIP
Rapid sample
aspiration
No events in
acquisition display
and RUN button is
green
Possible causes Recommended solutions
Sample tube other than
FalconĀ® tubes used
Use Falcon 12 x 75-mm sample tubes.
See Equipment (page 124).
Worn Bal seal Replace the Bal seal. See Changing the
Bal seal (page 58).
Sample tube is cracked Transfer contents to a new tube.
Possible causes Recommended solutions
Support arm is to the side Place the support arm under the
sample tube.
Droplet containment module
is failing
Try the solutions in Droplets are
visible on the SIP (page 102). If the
issue is not resolved, call your BD
service representative.
Possible causes Recommended solutions
Threshold is not set to the
correct parameter (usually
FSC)
Set the threshold to the correct
parameter for your application.
Threshold level is too high Lower the threshold level.
PMT voltage for threshold
parameter is set too low
Set the PMT voltage higher for the
threshold parameter.
Gating issue See the BD FACSDiva Software
Reference Manual for information on
setting gates.
Air in the sheath filter Purge the filter. See Removing air
bubbles (page 37).
Air bubble or debris in the
flow cell
Prime the fluidics system. See Priming
the fluidics (page 42).
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