User’s Guide104
No sample in the tube Verify that sample remains in the tube
and if necessary, add sample to the
tube or install a new sample tube.
Sample is not mixed properly Mix the sample to suspend the cells.
Waste tank is full Empty the waste tank.
PMT voltages set too low or
too high for display
parameter
Adjust the PMT voltages.
Too few events are displayed Increase the number of events to
display.
Sample injection tube is
clogged
Remove the sample tube to allow
backflushing.
If the event rate is still erratic, clean
the sample injection tube. See Cleaning
the fluidics (page 49).
Bal seal is worn Replace the Bal seal. See Changing the
Bal seal (page 58).
Instrument is not warmed up Wait 30 minutes for the instrument to
warm up.
Laser delay is set incorrectly Adjust the laser delay settings. See
Manual settings (page 111).
Laser is not functioning Verify the malfunction by changing the
threshold to an alternative laser while
running the appropriate sample. If
unsuccessful, contact BD Biosciences.
Tube is cracked or misshapen Replace the sample tube.
Possible causes Recommended solutions
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