User’s Guide114
5. Set an initial laser delay value only for the laser you are
optimizing.
• If you are optimizing the violet laser, set its delay to –35 µs.
• If you are optimizing the UV laser, set its delay to –65 µs.
• If you are optimizing the red laser, set its delay to 65 µs.
• If you are optimizing the blue laser, set its delay to 0 µs.
• If you are optimizing the yellow-green laser, set its delay to
35 µs.
6. While observing the positive events on the histogram, adjust
the laser delay in 1-µs increments. You might need to adjust
the delay above or below the initial setting.
Choose the setting that moves the events farthest to the right
(highest fluorescence intensity).
7. Draw an interval gate on the histogram for the positive events.
8. Create a statistics view to display the mean fluorescence
intensity (MFI) of the gated population.
9. While observing the MFI for the gated population, adjust the
laser delay in 0.1-µs increments within a range of 2.0
µs of the
setting obtained in
step 6.
Preserve the setting that maximizes the fluorescence intensity.
10. Reset the Window Extension to 10 µs.
To Next Page
Loading...
