BD FACSymphony A3 - Page 119

Chapter 8: Manual settings 119
• Decrease the area scaling factor if the FSC-A signal is
higher than FSC-H.
c. View the result of your change in the histograms and
statistics views.
12. Adjust the blue laser area scaling factor until the FITC-A signal
matches the FITC
-H signal, if needed.
13. Unload the FITC-positive control tube, then load the APC-
positive control
tube.