Chapter 4: Maintenance 53
7. Prime the instrument twice:
a. Press the PRIME button on the fluidics control panel.
b. When the STANDBY button lights (amber), press the
PRIME button again.
8. Install a tube with 3 mL of undiluted BD FACSClean solution
on the SIP and put the tube support arm underneath the tube.
See Maintenance overview (page 48) for other recommended
cleaning solutions.
9. Press RUN and HIGH on the cytometer fluid control panel.
Run for 30 minutes.
10. Press the STANDBY fluid control button and depressurize the
sheath container by lifting the vent valve.
11. Repeat steps 2 through 10 with BD FACSRinse solution.
12. Repeat steps 2 through 10 with DI water.
13. Replace the sheath filter and refill the sheath container with
sheath fluid.
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