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BD FACSymphony A5 - Page 57

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Chapter 4: Maintenance 57
Removing the old
filter
To remove the old filter:
1. Place the cytometer in standby mode.
2. Remove the sheath filter assembly by pressing the
quick-disconnect on both sides of the filter assembly.
3. Over a sink or beaker:
Remove the vent line from the filter and set it aside.
Remove the filter base and set it aside.
4. Discard the used filter assembly in an appropriate receptacle.
Attaching the new
filter
To attach the new filter:
1. Connect the vent line to the new filter assembly.
Twist to attach.
2. Wrap Teflon tape around the filter threads, then connect the
filter to the filter base.
3. Connect the sheath line to the filter assembly by squeezing the
quick-disconnect.
4. Attach the cytometer fluid line to the filter assembly via the
quick-disconnect.
5. Direct the vent line into a beaker and press the small button at
the end of the vent fitting against the side of the beaker until a
steady stream of fluid empties from the filter.
6. Tilt the filter and verify that no trapped air remains in the
filter.
7. Repeat steps 5 and 6 as necessary to remove all trapped air.

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