Biometra TProfessional Basic - Heating and Cooling Rate; Time Increment Δt(S)

40 Instruction Manual TProfessional Basic 06/2011
If options have been changed from the default values, there is an indication in the options field.
5.5.1 Heating and cooling rate
Since the TProfessional Basic is a fast thermocycler it may be necessary to reduce the heating
and cooling ramp to adapt protocols from slower thermocyclers.
The average heating and cooling rates can be set in the column labelled with Ê[°C/s].
Note: This entry specifies the speed at which the referring step is achieved
. I.e. to heat from
step 1 to step 2, the heating rate in step 2 has to be lowered.
Note: To reduce the over all speed of the Thermocycler, the heating rate has to be modified in
all steps. The speed settings will be only valid for this individual program.
5.5.2 Time increment Æ Δt(s)
To compensate for loss in enzyme activity, each step within a loop can be extended from cycle
to cycle. Enter the desired time increment [seconds] in the row labelled with dt[s]. This value will
be added to the time value from cycle to cycle.