SECTION 4
PREVENTIVE MAINTENANCE AND SAFETY
4.2 Cleaning and Decontamination Procedures
Chemically Disinfecting Single or Dual Chambers
1 Remove used Cytopads as described in Section 3.1.
2 Submerge chambers and frames in diluted (1/10) household bleach. Make
dilution fresh each day.
3 Soak for at least one hour.
4 Scrub interior chamber surfaces with detergent-soaked cotton-tipped swab to
remove residual cells.
Note: This procedure is effective against Hepatitis B (HBV) and HIV and is at least
partially effective against CJD. Treatment with sodium hypochlorite followed by 1N
sodium hydroxide for 1 hour is considered completely effective against CJD.
CAUTION!
Using sodium hydroxide on the rotor can cause serious damage.
5 Rinse thoroughly with deionized water and dry before reuse.
Autoclaving Single or Dual Chambers
1 Submerge the frame and chamber in any dilute detergent solution.
2 Remove frame and chamber from the detergent solution.
3 Autoclave for at least one hour at 132 °C for complete sterilization and certain
inactivation of CJD virus.
Note: While the Cytopro single or dual chambers are reusable, autoclaving
limits chamber life. Discard any chamber, frame, or cap that appears distorted
or will not fit with other components. Most chambers can be autoclaved up to
20 times without showing any signs of degradation. CYTOPRO MAGNUM
CHAMBERS ARE NOT REUSABLE. DO NOT ATTEMPT TO CLEAN OR REUSE.
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