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SECTION 4: SWEAT ANALYSIS
4.1 An overview of Sweat Analysis
The procedures described up to this point in the manual provide the laboratory technician with an
undiluted sweat sample. By virtue of the specific safeguards against condensation and
evaporation error, the sample is fully representative of the patient’s secretion and is therefore a
valid specimen for analysis as long as the sweat rate exceeded 1g/m
2
/min, or a sweat sample of at
least 15 µL in 30 minutes of collection. Results for samples less than 15 µL should not be recorded
as valid sweat tests, nor should insufficient sweat samples be pooled to achieve the volume
required.
Chloride Analysis
Sweat samples collected with the Macroduct Advanced can be analyzed for the sweat chloride
level. ELITechGroup provides the ChloroChek Chloridometer as an operator friendly method to
measure chloride levels in sweat. The ChloroChek Chloridometer is a coulometric titrator designed
to determine chloride ion concentrations in sweat samples in less than 20 seconds with just 10 µL
of sweat.
Electrical Conductivity
ELITechGroup’s Sweat-Chek Sweat Conductivity Analyzer was designed to measure conductivity of
Macroduct collected samples. Field testing in clinics in the U.S. and in many other countries
testifies to its simplicity, economy, and accuracy in the diagnosis of cystic fibrosis.
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