2. When observing a sample with weak fluorescence with the IR cut filter removed (or
without a sample), flare may be conspicuous that depends on the characteristics of
the mirror unit if light sources that use a mercury lamp are combined.
3. Restrictions
when
individual
products are
combined
1. Flare may be conspicuous if a filter with high reflectance (45LBD-IF, etc.) is inserted
into the filter holder of the microscope.
It will be less prominent by inserting an ND filter for transillumination or low-
reflection type ND filter (45-ND25, etc.) on the condenser side of the high-
reflectance filter.
3.2 Specifications subject to restrictions depending on usage conditions
3.2.1 Cameras in general
1. Because the pixel shift mechanism is sensitive to impact, avoid any impact to it when attaching it to or
removing it from the microscope. Be sure to use the dedicated packaging box when transporting this
product.
2. It is necessary to perform calibration periodically (approximately once every three months) for late-onset
pixel defects caused by cosmic rays. (operate with cellSens / PRECiV / DP2-TWAIN)
3. Do not use live images, captured images, or analytical images using these images for diagnostic purposes.
4. Pixel shift imaging of 8192 x 6000, 4096 x 3000 pixels (3CMOS mode) by external trigger input is not
available.
3.2.2 Camera images
1. Image disturbance such as a hatching pattern will occur when the specimen is moved during pixel shift
imaging with 8192 x 6000, 4096 x 3000 pixels (3CMOS mode).
2. Image disturbance such as a hatching pattern will occur if there is camera or microscope vibration during
pixel shift imaging with 8192 x 6000, 4096 x 3000 pixels (3CMOS mode).
Factors that cause vibration include operating the keyboard/mouse or equipment with a built-in air cooling
fan on the desk on which the microscope with the camera has been placed.
3. When the live noise reduction function is enabled, afterimages or blackened areas may occur when the
specimen (stage) is moved.
4. When using the live HDR function, afterimages will occur if the stage is moved.
5. With still image HDR, pixel shift imaging of 8192 x 6000, 4096 x 3000 pixels (3CMOS mode) is not available.
6. Auto white balance does not work properly with light sources other than halogen light sources or high
color rendering LED light sources.
7. Color reproduction is different before and after insertion of the IR cut filter.
8. Auto black balance may leave the background stood out depending on the observing conditions.
9. Scenes may not be determined correctly in scene recognition.
Specific examples:
l
when there is no specimen in the field of view, or the contrast of the specimen is low, or the image
is greatly out of focus, BF is erroneously determined as DIC/PH
l
when switching the optical path, erroneous determination of fluorescence observation is made
l
specimens for industrial use (examples: substrates, metal etc.)
10. Flashing defective pixels may appear during long-time live imaging.
8
3. Restrictions 3.2 Specifications subject to restrictions depending on
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