© Boule Medical AB, March 2018. Article no. 1504496 Page 75
8. Technology
Measurement of Eosinophils
Measurement of Eosinophils
4 part WBC dierential impedance based analyzer
• The method is included the conventional 3 part dierential analysis together with an added EOS
measurement with a total analyzing time of about 3 minutes.
• After the 3 part dierential analysis the instrument continues with an additional WBC dilution
but instead of lyse, EOS reagent is used to achieve the nal 1:400 dilution.
• After an incubation time all cells are lysed except the eosinophils.
• The EOS population is displayed as a separate size-distribution curve, see g below.
• A settable discriminator is used to separate the EOS population from debris and to count the total
number and percentage in relation to the total WBC count.
Figure 119: EOS Dierential
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