Program the concentration of the standard solution in the Span Calibration menu (2.3.2 Span
Calibration). The concentration of the calibration standard used must be typically greater than 50%
of the full scale of the RANGE the calibration is carried out. In order to see BioTector calibrated
ranges, see System Range Data screen (2.2.3 System Range Data Screen). (To prepare a standard
solution, see procedures described in Section 6.2 Calibration Standards or information sheet “R009.
Standard Solutions for BioTector Multi-component Analyzer”, which is available inside the MMC/SD card
shipped with the BioTector.)
Connect the standard solution to the MANUAL/CALIBRATION port. If these ports are not available,
use the SAMPLE 1 port. Avoid the manual purging of the calibration, manual grab sample and
sample lines using the Simulate menu, because the system reactor may get contaminated during
the automatic sample valve and pump synchronization process. To purge these lines, it is
recommended to use PUMP FORWARD TEST and PUMP REVERSE TEST functions in the
Sample Pump Test menu (see 8.1.1.4 Sample Pump Test for details). It is recommended that the
standard solution is located at the same height as the sample pump. Run the Span Calibration
cycle using the RUN SPAN CALIBRATION function in Span Calibration menu. A minimum of five
complete analysis cycles is recommended for the span calibration. Span Calibration cycle should
be carried out only when stable zero readings are obtained after a Zero Calibration cycle completed
without any warnings. (In systems using multiple operation ranges, it is recommended to carry out a Span
Check cycle at the other operation ranges used and if necessary to adjust the span factor manually using the
Span Calibration menu.)
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