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As can be seen in the figure 14 above, unlike all other reagents, base reagent container does not contain any
vent (breathing) hole. The breathing air into the base container is supplied through the CO
2
filter, which must
be fitted on the base container lid.
Figure 15 below shows the detailed connections on BioTector base reagent. The purpose of the CO
2
filter is
to prevent the base reagent coming in contact with atmospheric CO
2
present in the air. The soda lime inside
the CO
2
filter absorbs the atmospheric CO
2
and prevents the base reagent getting contaminated. If any vent
hole is accidentally drilled on the lid of the base container and if the fittings are not connected correctly on the
base reagent, contamination will occur and the background CO
2
readings will increase.
Figure 15 BioTector base reagent dip tube setup
All other reagent containers’ (except the base reagent container) lids must contain a 3mm vent hole. Failure
to do so may cause the container to collapse and leak.
The length of the dip tubes in all reagent containers should be adjusted correctly for the optimum usage of the
reagents. Stainless steel (SS-316) weights should not be used in any reagent which contains HCl acid. The
recommended weights for such reagents are PFA.
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