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To store the program, press
ENTER. The display will automatically
return to the Create Program display. If you do not wish to store the
program, press
EXIT. If corrections are needed, see section 3.11 Editing
User-Entered Programs.
If only one calibration standard is measured, a straight line between
that point and the origin (0 Abs, 0 Conc) is calculated, and
measurements are linearly interpolated along that line.
If two or more data points (up to a maximum of 45) are entered, you
can choose a piecewise or linear equation for converting absorbance
to concentration.
If four or more data points are entered, you can use a piecewise, linear
or quadratic equation for converting absorbance to concentration.
If five or more data points are entered, you may use a cubic equation
or any of the other equations mentioned to convert absorbance
to concentration.
To use a user-stored program, either scroll to the program or enter the
program number with the keypad. Any timing intervals stored in the
program must be observed when testing samples with the method.
If the concentration of a sample exceeds the high limit, the display
flashes a warning of the over-range condition. Repeat the method
using a fresh sample that is diluted to fall within range of the
calibration curve.
If the concentration of the sample is less than the low limit, the
display flashes a warning of the under-range condition.
3.11 Editing User-Entered Programs
All program option information stored previously by the operator can be
reviewed and changed to add, delete or modify data points. At any point
during the editing function, the operator can terminate the procedure and
exit by pressing
EXIT. The display will prompt Store Changes? Press ENTER
to store the changes and exit. Press EXIT to exit without storing changes.
If desired, you may modify only the calibration table for new lots of
reagents or changes in the sample matrix (improves accuracy). All the
other options of the calibration can remain the same. If you are not sure of
making changes to a user program, copy the original program (see section
3.13). If the changes are not desired the original program is still intact and
may be used.
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