Microlab
®
STAR User Manual
Page 16 of 225 610766/02
'LVSHQVH
The transport air that was aspirated at the end of the aspiration step is first dispensed with
the tip still in the air. Dispensing of the liquid may now occur in two different modes: to the
liquid surface or in a jet.
To ensure that the specified accuracy is achieved, volumes below 20 µl should always be
GLVSHQVHGWRDOLTXLGVXUIDFH. For dispensing to a liquid surface use cLLD to detect the
position of the surface and then dispense by following the rising liquid level. For volumes
larger than 20 µl the liquid can be GLVSHQVHGLQDMHW without touching the surface. To
dispense in a jet specify a position a couple of millimeters above the surface and dispense by
following the rising liquid level. For dispensing in a jet, a varying amount of blowout air is
used to make sure that all liquid is dispensed from the tip.
However, dispensing to the surface of an empty vessel (touch off) is also possible For
dispensing into an empty container, position the tip less than 1 mm (typically 0.4 mm) above
the bottom of the container and dispense, following the rising liquid level.
After the dispense, the situation within the tip looks like this:
6LWXDWLRQRQGLVSHQVH)LUVWO\WUDQVSRUWOLTXLGDQGEORZRXWYROXPHDUH
GLVSHQVHGIROORZHGE\DQDVSLUDWLRQRIVWRSEDFNYROXPHLQWKHFDVHRI
SDUWLDOYROXPHGLVSHQVHVDQGWUDQVSRUWDLU
Dispense in Jet Dispense to Surface
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