Hamilton Microlab STAR - Aspiration; Dispense

Microlab
®
STAR User Manual
Page 18 of 225 610766/02
The OLTXLGFODVV stores all relevant background parameters, such as flow rates and volume
corrections, for one pipetting cycle, i.e. for one aspiration and the subsequent dispense(s).
Depending on the pipetting mode chosen, only a subset of the parameters of the liquid class
is active. According to the different dependencies listed above, liquid classes have attributes
related to their intended use: tip type, liquid name, and dispense mode.
Different liquid classes are provided with the User Software and optimized for different
liquids, tip types, and important pipetting processes, such as aspiration followed by
dispensing either to a surface or in a jet. HAMILTON has optimized the standard liquid
classes with great care to assure the best pipetting accuracy. To change HAMILTON
standard liquid classes, store the class under a different name first. For special applications,
the user can define his or her own liquid class to achieve the highest accuracy with the
compounds and volumes of interest. For this purpose a Liquid Editor comes with the
Microlab STAR User Software. It is described in Chapter 11 below.
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XQFRQWUROOHGUHVLGXDOYROXPHVPD\EHOHIWZLWKLQWKHWLSRURWKHUHUURUVUHODWLQJWRWKHVXPRI
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Here are some examples of frequently-used combinations of liquid classes and pipetting
modes:
$VSLUDWH≥µORIDZDWHUOLNHOLTXLGGLVSHQVHµOLQWRDQHPSW\ZHOOSODWHXVH
VWDQGDUGWLSVFKDQJHWLSVHYHU\F\FOH:
Liquid Class: StandardVolume_Water_DispenseJet
Aspiration Mode: Aspiration
Dispense Mode: Jet Dispense Empty Tip
Detection: Aspiration: LLD = pressure or capacitance or both, submerge to a
depth of 2mm, following liquid level
Dispense: Fixed height of 5mm, not following liquid level
$VSLUDWHDZDWHUOLNHOLTXLGVLQJOHGLVSHQVHLQWRDSUHILOOHGZHOOSODWHXVHORZ
YROXPHWLSVFKDQJHWLSVHYHU\F\FOH:
Liquid Class: LowVolume_Water_DispenseSurface
Aspiration Mode: Aspiration
Dispense Mode: Surface Dispense Empty Tip (in the liquid class selected here, the
blowout volume is 0)
Detection: Aspiration: LLD = Pressure or Capacitance or both, Submerge Depth
2mm, following liquid level
Dispense: Capacitance LLD on, following liquid level