Microlab
®
STAR User Manual
610766/02 Page 19 of 225
$VSLUDWH≥µORIDZDWHUOLNHOLTXLGGLVSHQVHWKHVDPHDPRXQWLQWRDQHPSW\
ZHOOSODWHNHHSWLSV
Liquid Class: StandardVolume_Water_DispenseJet
Aspiration Mode: Aspiration
Dispense Mode: Jet Dispense Empty Tip (empty tip only)
Detection: Aspiration: Capacitance LLD, submerge depth 2mm, following liquid
level
Dispense: Fixed height of 5mm, not following liquid level
Comment: On first aspiration, pre-wetting of the tip by 1-3 mixing cycles is
necessary to equalize conditions for initial and subsequent dispenses.
$OLTXRWLQJ of liquid means aspirating a given volume all at once and dispensing several
partial volumes (aliquots) in a jet to different containers. In this frequently-used pipetting
procedure, measurements have revealed that the accuracy of the first and the last aliquot are
often not within the specified range. Therefore, to dispense e.g. 10 aliquots of 20 µl of a
ZDWHUOLNHOLTXLG with the ML-STAR, aspirate 240 µl and dispense 20 µl directly back into the
container. This is followed by dispensing 10 of the 20 µl aliquots. The last aliquot of 20 µl is
discarded into another container or ejected with the tip. In addition, after the dispense of
every aliquot, a given amount of air is aspirated and dispensed with the next aliquot.
Liquid Class: StandardVolumeWaterAliquotJet
Aspiration Mode: Aspiration
Dispense Mode: Jet Dispense part volume
Detection: Aspiration: Capacitance LLD, Submerge Depth 2mm, following liquid
level
Dispense: Fixed height of 5mm, not following liquid level
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