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HEKA EPC 9 User Manual

HEKA EPC 9
101 pages
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8.52
EPC 9 Manual
HEKA Elektronik Dr. Schulze GmbH
Wiesenstraße 71 • D-67466 Lambrecht • Germany
Tel: +49 (0) 6325 9553 0 • Fax: +49 (0) 6325 9553 50
Web Site:
http://www.heka.com
E-mail:
sales@heka.com
support@heka.com

Table of Contents

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HEKA EPC 9 Specifications

General IconGeneral
BrandHEKA
ModelEPC 9
CategoryLaboratory Equipment
LanguageEnglish

Summary

Installation

Calibrating the EPC 9

Guides users through the amplifier calibration process using E9SCREEN software for optimal performance.

Creating the C-fast Lookup Table

Explains how to generate a C-fast lookup table for automatic compensation in E9SCREEN and PULSE.

Verifying and Testing the EPC 9

Testing the EPC9 with the Model Circuit

A tutorial on using a model circuit to test EPC9 amplifier functions and verify operation.

Making a "Full Test"

Describes a diagnostic tool in E9SCREEN for comprehensive hardware testing and troubleshooting.

Measuring the Frequency Response

Explains how to use Test mode to measure the EPC9's current-measuring circuitry and frequency response.

E9SCREEN Software

Operating Modes

Voltage-Clamp Mode

Describes the basic patch-clamp mode controlling pipette potential and converting current to voltage.

Current-Clamp Mode

Explains how feedback is used to maintain current at zero by varying pipette potential.

Compensation Procedures

Series Resistance Compensation

Explains the importance and method of compensating for series resistance errors in voltage-clamp recordings.

Offset Compensation

Covers handling and correcting various offsets (amplifier, electrode, junction potentials) during patch-clamp experiments.

Patch-Clamp Setup

Using the Patch Clamp

Forming a Seal

Details the steps for initial setup, entering the bath, and achieving a seal with a cell.

Cell-Attached Recording

Explains how to perform cell-attached recordings, adjust controls, and set parameters.

Whole-Cell Recording

Covers breaking the patch, capacitive transient cancellation, and series resistance compensation for whole-cell mode.

Current-Clamp Recording

Describes switching to current-clamp mode and applying stimulus pulses for recording membrane potential.

Low-Noise Recording