5-54 Echo Liquid Handler User Manual
If the source plate fluid is stable, the survey interval can be safely increased.
For example, “every 5 plates” will cause a new survey to be performed
when the source plate and first destination plate are loaded, and every fifth
destination plate thereafter. Increasing the survey interval may improve
throughput.
Default: 1.
Note: The survey interval can be set to 0; however, with this setting
the protocol will not survey the plate.
Assume Homogeneous Plate: When a
source plate is surveyed to measure the
DMSO/water ratio in each well, the Echo
liquid handler creates a DMSO concentration map of the entire plate. This
map is post-processed using a moving average filter if the ASSUME
HOMOGENEOUS PLATE checkbox is selected. There is no overhead associated
with this processing. Typically, better liquid transfers are obtained using
post-processing when the source microplate contents are homogeneous. A
homogeneous source microplate is one where the solution to be
transferred is very similar, well-to-well.
If the USE PREDEFINED checkbox is selected, the Assume Homogeneous
Plate feature is unavailable, and its checkbox is “grayed out.”
Default: Assume homogeneous plate off.
Power Monitoring: Selecting the POWER
MONITORING checkbox causes the Echo liquid handler
to monitor the RF power used to eject each fluid droplet. Labcyte
recommends that power monitoring not be enabled by the user unless
directed to do so by Labcyte personnel. Enabling power monitoring adds
significant overhead to each droplet ejection and decreases liquid transfer
throughput.
Default: Power monitoring off.
Save Plate-Survey Data to Disk: The data
collected during a source plate survey is held in
cache memory. It is replaced when the next
source plate survey is performed. If the SAVE
PLATE-SURVEY DATA TO DISK checkbox is selected, the survey data will be
stored on the client PC disk. The data file is stored to <InstallDir>\Labcyte\
Medman\Data. This data can be useful to Labcyte personnel during
diagnostic troubleshooting.
Default: Source plate survey data is not saved on the Echo controller.
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