3-22 Echo Liquid Handler User Manual
3.3.8 Supported labware
Source microplates: The following Echo qualified source microplates are
available:
Table 3.3 DMSO Source Plate Types
Plate Calibration Vol. range (μL)
Working vol.
(μL)
384PP 384PP_DMSO2 15-65 50
384LDV 384LDV_DMSO 2.5-12 9.5
1536HB 1536HB_DMSO 2- 6 4
1536LDV 1536LDV_DMSO
1536LDV_DMSO_HT
1
1.0 - 5.5 4.5
1
HT=High Throughput. This calibration is available on Echo 555 models only. It uses the same
1536LDV plate, but with a faster transfer rate. For transfer of higher volume or where precise
droplet placement is not required, see “1536-plate to 1536-plate liquid transfer” on page 1-17.
Table 3.4 Aqueous Source Plate Types
Plate Calibration Vol. range (μL) Working vol. (μL)
384PP 384PP_AQ_BP2
(buffer w/protein) 15 - 65
50
384PP_AQ_SP2
(buffer w/surfactant &
protein)
15 - 65
384PP_AQ_CP
1
(dynamically adjusts for
surface tension/viscosity)
25-50 25
384PP_AQ_GP2
(aqueous w/glycerol,
from 25 to 50% volume)
15 - 65
50
384LDV
2
384LDV_AQ_B
(buffer)
3 - 12 9
384LDV_AQ_BP2
(buffer w/protein)
6 -14 8
1
Volume range 15-65 is the working range but performance for sparse matrix
crystallography sets will be more optimal in the 25-50 ul range.
2
Plate 384LDV aqueous plate precision and accuracy are only guaranteed for a
maximum depletion of 500 nL from a single well without resurveying. The software
will present a warning message when the depletion exceeds 500 nL, but will allow you
to continue. Labcyte recommends that you test the performance of any single well
depletion greater than 500 nL without a re-survey.
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