3-20 Echo Liquid Handler User Manual
3.3.7 Fluid transfer
Volumetric Accuracy: <10% error (single source plate-wide average from
target volume)
1
Volumetric Precision: <8% CV
DMSO/water concentration: <8% accuracy error; <5% precision CV
(Echo 550 and 555 models only)
Fluid temperature: 21°C ± 5°C (70°F± 9°F)
Compound should be in liquid form prior to transfer.
Throughput: Transfer time for fluids in different plates and on different
Echo systems.
1
Plate calibration 384PP_AQ_CP works with a much wider range of fluids. There are use
cases (particularly in protein crystallography applications) where the volumetric accuracy
may deviate from this specification. Please contact your Labcyte account representative
with any questions.
Table 3.2 Fluid throughput comparison
Plate type
1
Transfer
volume (nL)
Echo 555
(min)
Echo 550
(min)
Echo 520
(min)
1 384PP_DMSO2 2.5 1.4 2.5 2.5
2 384PP_AQ_BP2 2.5 1.8 2.9 --
3 384PP_AQ_CP 2.5 2.7 3.7 --
4 384PP_AQ_GP2 2.5 1.8 2.9 --
5 384PP_AQ_SP2 2.5 1.7 2.7 --
6 384LDV_DMSO 2.5 1.5 2.6 2.6
7 384LDV_AQ_B 2.5 1.5 2.6 --
8 1536LDV_DMSO 2.5 2.6 4.5 4.4
9 1536LDV_DMSO_HT 2.5 2.6 -- --
10 1536HB_DMSO 2.5 3.1 5.0 5.0
1 384PP_DMSO2 100 1.9 3.8 7.7
2 384PP_AQ_BP2 100 3.1 4.2 --
3 384PP_AQ_CP 100 6.0 7.2 --
4 384PP_AQ_GP2 100 3.1 4.2 --
5 384PP_AQ_SP2 100 2.2 3.3 --
6 384LDV_DMSO 100 2.0 3.9 7.7
7 384LDV_AQ_B 100 2.8 3.9 --
8 1536LDV_DMSO 100 5.8 8.2 12.7
9 1536LDV_DMSO_HT 100 4.7 -- --
10 1536HB_DMSO 100 6.2 10.2 25.5
1
These plate type names are used with instruments that have shipped with Echo Server 2.4. Instruments that
shipped with earlier versions of software may use a different naming terminology and may have different
transfer specifications. Contact your Labcyte account representative with any questions for your specific
instrument.
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