4-10 Echo Liquid Handler User Manual
4.3 Create liquid transfer protocol
The liquid transfer protocol specifies how much fluid will be transferred
from the source microplate to the destination microplate, and in what well
order
—a plate map that the Echo instrument uses to perform the transfer.
For example, a simple transfer protocol would transfer compound from all
source wells to the matching destination wells, replicating the contents of
the source plate. More complex protocols would transfer compound from
different source plates to one or more destination microplates. The Echo
liquid handler software provides a plate map to specify the wells that will
be used in the liquid transfer. For a detailed discussion of different types of
liquid transfer protocols, see “
What types of liquid transfer are
supported?” on page 1-12.
Note: To create transfer protocols for specific applications or
specialized plate configuration, such as dose-response or protein
crystallization, you can purchase optional software applications or the
Echo Software Development Kit to create your own application.
See “Echo applications” on page 1-19.
Sample protocol parameters
The example in the following procedure uses these parameters:
Source microplate:384LDV_AQ_P
(384-well, COC, low dead volume microplate for aqueous protein
solution)
Destination microplate: An empty 384LDV microplate
Plate map: The twenty wells defined as A1 through E4
Copies: 5
Volume: 10 nL transfer into each well
Source well contents: Sample fluid consisting of 1 mg/mL protein
diluted in aqueous buffer.
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