Labcyte Echo 555 - Page 103

Echo Liquid Handler Software 5-3
Note: See “What types of liquid transfer are supported?” on
page 1-12 for definitions of plate mapping terms.
Examples of protocols that cannot be created with the standard Echo
software included with the Echo liquid handler installation:
 Transfers other than replication
*
. For example, well A1 into well C12.
 Variable drop volume transfers. For example, 5 nL from source well
A1 to destination well A1, 10 nL from source well A2 to destination
well A2, and so on.
 Transfers to non-standard destination plates.
Note: Complex and non-standard liquid transfer protocols can be
created by using the Echo Cherry Pick and Echo Plate Reformat
applications, or by programming your own custom protocols with the
Echo ActiveX programming interface (refer to the Labcyte Echo
Integration Manual).
Table 5.1 Liquid transfer types
Source
plate
Destinatio
n plate
Transfer map type
(full or partial)
384-well 96-well
destacking
1
quadrant
(4 regions from 1 src plate to 4 dest plates)
destacking interleaved
(2x2, or every other dest well)
384-well
384-well standard (1 src plate to 1 dest plate)
384-well 1536-well
stacking
2
quadrant
(4 src plates to 4 regions on 1 dest plate)
stacking interleaved
(2x2, or every other dest well)
384-well 3456-well
stacking quadrant
(9 src plates to 9 regions on 1 dest plate)
stacking interleaved
(3x3, or every third dest well)
1536-well 96-well
destacking quadrant
(16 regions from 1 src plate to 16 dest
plates)
destacking interleaved
(4x4, or every fourth dest
well)
1536-well 384-well
destacking quadrant
(4 regions from 1 src plate to 4 dest plates)
destacking interleaved
(2x2, or every other dest well)
1536-well
1536-well standard (1 src plate to 1 dest plate)
1 Destacking: transferring liquid from a single source (src) plate to several destination (dest) plates
2 Stacking: transferring liquid from several source plates to a single destination plate
* Compound from the source plate wells are transferred to the same relative well posi-
tions in the destination plate. For example, transferring compound from the first two
columns in the source plate to the first two columns in the destination plate.