5-50 Echo Liquid Handler User Manual
5.4.3 Transducer calibration
There are two acoustic transducer calibration tools:
Focus calibration measures the
focal length of the acoustic
transducer and compares it to the
previous measurement. If no
significant focal length change has
occurred, no recalibration is required and the focus calibration
procedure is finished.
Focal-Sweep is a diagnostic tool used by Labcyte personnel to
measure the focal length of the acoustic transducer during a
predefined vertical movement.
Focus calibration
The Echo acoustic transducer includes a lens that focuses the acoustic
energy pulse at a specific point near the microplate well fluid surface.
Correct droplet ejection requires the ability to accurately focus the acoustic
energy in the well fluid.
Changes to the transducer focal length over time may lead to a
mispositioning of the Z-axis. Measurement of the DMSO/water ratio during
a source plate survey will be affected. In turn, inaccurate DMSO/water ratio
measurement can degrade the accuracy of fluid droplet ejection.
Calibrating the Echo acoustic transducer system compensates for certain
changes in the transducer that can accumulate over time and use.
Focus calibration check
A focus calibration check should be performed periodically. The Echo liquid
handler keeps track of how long it has been since the last focus calibration
check was performed. When a new focus calibration check should be
performed, the F
OCUS CALIBRATION indicator—on the DIAGNOSTICS tab—
will turn from green to yellow.
Figure 5.46 System Status section
To Next Page
Loading...
