Leica DMIL - Adjusting the Slit-Diaphragm

48
Attention:
Do not loosen the screws (49.4) on the slide!
• The line of light must be fully located on the
grey field. With the 10x objective, the image of
the modulator and of the line of light have
almost the same size. Adjust the slit-
diaphragm so that the boundary of the bright
line of light is located near the darker edge.
• Move the other objectives with ascending
magnification into the light path one after the
other and check the position of the line of
light. In the case of smaller deviations, find an
intermediate position.
Always make sure that the objective magni-
fication and the slide position on the IMC slit-
diaphragm slide match.
!
Positioning the IMC slit-diaphragm slide on the
transmitted-light illumination carrier
• Remove the empty slide or the phase contrast
slide in the transmitted-light illumination
carrier, if present.
• Hold the diaphragm holder so that the
lettering “Top left“ is located on the top left
side and the other lettering points forward.
The catches are located on the upper long
side of the slide and point toward the centre
of the specimen stage.
• Insert the diaphragm slide from the right side
into the transmitted-light illumination carrier.
• Lock the slide in position IMC (lettering IMC
visible).
Adjusting the slit-diaphragm
• Fully open the aperture diaphragm.
• Select a medium brightness since the line of
light would otherwise appear too bright.
• Switch off any filters which may be switched
on.
• Rotate the objective with the lowest magnifi-
cation into the beam path, which will usually
be the 10x objective.
• To adjust the slit width, move the slide of the
IMC slit-diaphragm slide to the proper position
for the objective, e. g. to the position marked
10x for the 10x objective.
• Remove one eyepiece and insert the focusing
telescope.
• The line of light will appear as a bright line on
the grey image of the modulator. Focus the
line of light with the focusing telescope.
• Adjust the position of the line of light with the
adjusting screws to the right of the IMC slit-
diaphragm slide. A fitting Allen key is supplied
for this purpose.
Fig. 49 IMC components
1 IMC modulator, 2 IMC slit-diaphragm slide, 3 Catches,
4 Screws
1
3
2
4