LeadCare
®
II User's Guide 1-13
CAUTION: When preparing blood samples for testing:
• Wear powder-free gloves to prevent lead contamination.
Because there is lead in the environment, it is easy to
contaminate blood samples, collection tubes, and test kit
items. Contamination of the work environment can cause
inaccurate blood lead test results.
• Use only the heparinized capillary tubes provided with the
LeadCare II Test Kit. The capillary tube must be filled to the
fill line (50 µl) for accurate results. Check to make sure that
the tube is free of gaps and bubbles. After collection, wipe
off the sides of the capillary tube with a gauze pad (wipe
downward). The accuracy of the test depends on a precisely
measured sample.
• Use only fresh, unrefrigerated, whole blood with the
LeadCare Treatment Reagent. Do NOT refrigerate the blood
prior to mixing with the reagent. Blood must be stored at
10
o
- 32
o
C (50
o
- 90
o
F).
• Add blood sample to the treatment reagent within 24 hours
of collection. Blood older than 24 hours may produce false
negative results. Make sure the blood sample is free of blood
clots, which can cause inaccurate results.
• Visual Impairment: Any visual impairment, such as color
blindness may affect the operator's ability to detect the
sample color change. Operators with vision deficiencies
should invert the tube 8 to 10 times to ensure that the
sample is properly mixed.
Precautions for Testing a Patient Sample
CAUTION: When testing blood samples:
• Wear powder-free gloves to prevent lead contamination.
Because there is lead in the environment, it is easy to
contaminate blood samples, collection tubes, and test kit
items. Contamination of the work environment can cause
inaccurate blood lead test results.
• Do NOT allow the inside of the treatment reagent vial or the
vial cap to touch anything. This could cause inaccurate blood
lead test results.
• Mix the blood sample with the treatment reagent thoroughly,
but do NOT shake the tube. Gently invert the tube ten times
until the reagent turns brown. Avoid foam and air bubbles.