PIPETTOR OVERVIEW
9
Program Keys:
[Fill]
[Disp]
[Mix]
The Fill, Dispense, and Mix keys program the
pipetting operations. By pressing one of these keys,
you can enter the programming mode of the IMPACT.
The "Run" prompt disappears from the display and
the appropriate operation appears with a flashing
"Vol" prompt. Fill, dispense, or mix volumes can be
programmed by pressing the Volume key. Also, the
number of mix cycles can be programmed by
pressing the Mix key twice.
Automatic blowout. At the end of a final dispense
step, the pipettor automatically expels any remaining
liquid from the pipet tips with a short burst of air
(blowout). The pistons then reverse direction and
return to zero position.
Note: If you press and hold in the trigger during the
final dispense step, the pipettor performs the
automatic blowout and holds its position until you
release the trigger. This delay allows you sufficient
time to remove the tips from the liquid to prevent
aspiration of liquid, as the pistons return to zero
position.
s
t
Vol
IMPACT
2
only
The Volume key controls the quantity of liquid to be
aspirated or dispensed. The volume can be entered
when the "Vol" prompt appears.
Pipetting volume can be incremented quickly by
continuously depressing the Volume key. Short,
quick, depressions of the Volume key will increase or
decrease the volume by its smallest increment.
Longer depressions of the Volume key will increase
or decrease the volume in larger increments. Three
levels of volume increments are available to achieve
the desired volume (see Table C.1, Default Program-
ming Volumes).
The Volume key can also be used to change
pipetting speeds within a stored program or during
the execution of a program (see Section C.2.4,
Pipetting Speed Adjustment).
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