Matrix Impact2 - F. Appendices; Additional Pipetting Examples; Sample Transfers

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F. APPENDICES
F.1 Additional Pipetting Examples
F.1.1 Sample Transfers
Example #1: Transfer 100 µl and mix 200 µl for 2 cycles
immediately after transfer.
[Fill]
s
t
Vol
(100) [Enter]
[Purge] [Enter]
[Mix]
s
t
Vol
(200) [Mix]
s
t
Vol
(2) [Enter] [Enter] [Enter]
When a purge is programmed before a mix cycle, the pipet tips
are emptied and mixing occurs automatically without pressing the
trigger. At the end of the mix, "ZERO" flashes in the display.
Remove the pipet tips from the liquid and press the trigger to
return the pistons to zero position.
Example #2: Mix 250 µl for 3 cycles, then transfer 150 µl.
Option 1 Option 2
[Mix]
s
t
Vol
(250) [Enter] [Fill]
s
t
Vol
(150) [Enter]
[Fill]
s
t
Vol
(150) [Enter] OR [Mix]
s
t
Vol
(250) [Enter]
[Disp]
s
t
Vol
(150) [Enter] [Enter]
[Purge] [Enter] [Enter]
Option 1 requires an additional trigger press at the end of the mix
to zero the pistons (remove tips from liquid before zeroing).
Note: The mix cycle default is 3 cycles and does not need to be
programmed — unless it was changed in a previous program.
Option 2 minimizes the number of trigger presses and executes a
dispense by using the [Purge] key. First, fill the assigned volume
of liquid, then leave the tips in the liquid and execute the mix step.
Finally, transfer 150 µl by using the purge step.
Note: The mix step always returns to its starting volume;
therefore, the volume will be 150 µl at the end of the mix step.