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D. CALIBRATION
The IMPACT and IMPACT
2
are factory-calibrated for distilled water
at 20°C. For easy calibration, using distilled water, the IMPACT
2
pipettor features an “EASY” CAL mode (see Section D.2,
Calibrating the IMPACT
2
). When pipetting liquids of significantly
different specific gravities or temperatures, however, the Calibration
(CALC) feature may be used for both the IMPACT and IMPACT
2
pipettors to achieve greater accuracy (see Section D.1, Calibrating
the IMPACT).
To prepare for calibration, ensure that the pipettor and tips are at
room temperature. When calibrating the pipettor, select only one
channel to pipet with. Using an analytical balance, first obtain the
actual value of the programmed volume at factory calibration. The
programmed volume (Vp) used is typically the full volume of the
pipettor (for example, 250 µl for the 250 µl 8-channel pipettor), but
is not limited to this volume.
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