PROGRAMMING AND OPERATION
25
C.5 Operating Procedures
After you have programmed your pipettor, you are ready to begin
pipetting. The display should show the first program step, with the
"Run" prompt showing in the lower left corner:
The following steps describe a basic program for pipetting reagent
into a microtiter plate. The pipettor is programmed as follows:
[Fill]
s
t
Vol
(250) [Enter]
[Disp]
s
t
Vol
(30) [Enter] [Enter]
1. Attach pipet tips. When you are ready to begin pipetting,
attach the pipet tips to the pipettor.
2. Aspirate liquid. Immerse the pipet tips into the liquid to be
aspirated, then press the trigger to aspirate 250 µl.
3. Dispense liquid. Position the pipet tips over the first column
of wells in the microtiter plate, and press the trigger to
dispense 30 µl. At the completion of the dispense step, the
pipettor emits a single beep tone (if the beep tone is on).
4. Repeat dispense step. Repeat the previous step for the rest
of the columns in the microtiter plate. At the completion of the
eighth dispense, the pipettor emits a double beep tone (if the
beep tone is on, otherwise it emits a single beep tone) and
displays the "Purge" prompt. There is insufficient volume for
another 30 µl dispense, and the pipettor signals for a purge
step.
5. Discard remaining liquid. Position the pipet tips over a
waste container and press the trigger to purge the remaining
liquid. The pipettor emits a triple beep tone to signal the end of
the program, then returns to the first step of the program.
6. Eject tips. Press the ejector trigger to eject the used pipet
tips.
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