CALIBRATION
28
D.1 Calibrating the IMPACT
D.1.1 Volume Measurement
To measure the actual value of the programmed volume:
1. Fill the programmed volume (Vp), then dispense the entire
volume into a container on the analytical balance.
2. Read the weight on the analytical balance. Repeat this
procedure ten times.
Note: Use a clean and dry pipet tip with each pipetting cycle.
When filling the liquid, immerse the pipet tip approximately
¼ inch (approx. 0.6 cm) below the liquid surface. When
dispensing the liquid, touch the side of the container to ensure
a complete dispense.
3. Determine the average weight of the programmed volume and
convert it to volume. To convert to volume, correct the weight
for specific gravity and temperature.
If you are weighing water, use one of the correction factors
below:
Temperature Correction Factor
20°C - 22.5°C 1.003
23°C - 25°C 1.004
The resulting volume is the corrected actual volume. If the
pipettor is correctly calibrated, the programmed volume should
equal the actual volume (within the specifications of the
pipettor).
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