Matrix Impact2 - Calibrating the IMPACT 2; EASY CAL Mode

CALIBRATION
31
D.2 Calibrating the IMPACT
2
IMPACT
2
users can calibrate their pipettors with distilled water
using the “EASY” CAL mode or with other liquids using the original
“CAL” mode (displayed as “CALC” on the IMPACT
2
).
D.2.1 “EASY” CAL Mode
IMPACT
2
users can quickly calibrate their pipettor by using the
unique “EASY” CAL feature. “EASY” CAL has been developed for
fast, easy, in-lab verification and/or calibration using distilled water
at room temperature (20-22.5°C).
1. Weigh out ten aliquots of distilled water on an analytical
balance, using one pipetting channel. The programmed
dispense volume must correspond to the calibration point for
that pipettor (for example, 200 µl for a 250 µl pipettor). Refer to
Table D.1.2, Calibration Specifications, for proper calibration
points.
Note: Ensure that the distilled water used is at room
temperature (20-22.5 °C). An internal correction factor of
1.003 is used by the pipettor to convert weight (gm) to volume
(µl).
2. Calculate an average for the ten readings of distilled water
obtained from the balance. If the resulting average weight is
within your accuracy specifications, no further calibration is
required. If the average weight does not meet expected
performance, enter the “EASY” CAL mode to quickly re-
calibrate the IMPACT
2
.
3. Enter the “EASY” CAL mode by pressing the Special Function
key (using a pipet tip) until “EASY” flashes, then press the
[Enter] key to display the calibration point.
4. Use the [Vol] key to adjust the displayed calibration point
until it matches the average weight obtained in step 2. Store
this number by pressing the [Enter] key. The new calibration
setting is confirmed by the “CAL” and “SET” messages on the
display.