Matrix Impact2 - Serial Dilutions; Simple Dilutions

PROGRAMMING AND OPERATION
23
C.4.3 Serial Dilutions
Example: Transfer 100 µl to the first column of a plate previously
filled with reagent. Mix 200 µl for 2 cycles. Transfer 100 µl to the
second column and repeat the mix step. Follow this procedure for
the rest of the plate.
[Fill]
s
t
Vol
(100) [Enter]
[Mix]
s
t
Vol
(200) [Mix]
s
t
Vol
(2) [Enter] [Enter]
That's the entire program! The mix step always returns to the
volume at which it started (100 µl); therefore, the pipettor
continues the mix step indefinitely, until you press the [Purge]
key to end the program. In this example, the mix cycle is
programmed for 2 cycles (default is 3 cycles).
C.4.4 Simple Dilutions
Example: Prepare 1:10 dilution with a 10 µl sample volume.
[Fill]
s
t
Vol
(90) [Enter]
[Fill]
s
t
Vol
(20) [Enter]
[Fill]
s
t
Vol
(10) [Enter]
[Purge] [Enter] [Enter]
The first fill volume is for the diluent (90 µl), followed by an air-gap
(20 µl), and finally by the sample volume (10 µl). The purge step
will dispense the total volume.