APPENDICES
41
F.1.4 Simple Dilutions
Example: Prepare a 1:21 dilution with a 10 µl sample volume.
Dispense the total volume into a column and mix immediately.
[Fill]
s
t
Vol
(200) [Enter]
[Fill]
s
t
Vol
(20) [Enter]
[Fill]
s
t
Vol
(10) [Enter]
[Purge] [Enter]
[Mix]
s
t
Vol
(150) [Enter] [Enter]
This program contains an air-gap (20 ul) and mix step at the end.
The mix step begins immediately after the purge, without pressing
the trigger. Then the pipettor empties the pipet tips and displays
"ZERO." Remove the tip from the liquid, and press the trigger to
return the pistons to zero position.
Note: Whenever a purge step is immediately followed by a mix
step, mixing will occur automatically, without pressing the trigger.
F.2 Chemical Compatibility
With the IMPACT family of pipettors, operators can confidently
pipet acidic solutions or stopping agents without damaging the
pipet tip fittings. All pipettors are equipped with chemically-
resistant tip fittings which can withstand the corroding effects of
any acidic solution. Also, the IMPACT tip fittings can be wiped with
a bleach solution (5% sodium hypochlorite) for decontamination.
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