CALIBRATION
29
D.1.2 Pipettor Calibration
To calibrate the pipettor:
1. Calculate display volume of the pipettor: D
v
=
V
p x C
p
V
a
V
p
= Programmed Volume
V
a
= Corrected Actual Volume (corrected for temperature)
C
p
= Calibration Point (each pipettor handle has its own
calibration point, see the table below)
D
v
= Display Volume
Handle
Size
Calibration
Point (µl)
Factory
Calibration
Steps
12.50 µl
10.00 268
125.0 µl
100.0 483
250.0 µl
200.0 375
850 µl 700 882
1250 µl
1000 845
Table D.1.2: Calibration Specifications
2. Enter the CALC mode on the pipettor by pressing the Special
Function key (use a pipet tip to press the key) until the
“CALC” function flashes on the display, followed by the
calibration point.
3. Press the [Vol] key to enter the Display Volume (D
v
)
calculated above.
4. Press the [Enter] key to set the calibration point. The display
shows “CAL” then “SET” to confirm the new calibration point,
and returns to the current pipetting program.