APPENDICES
40
F.1.2 Incremental Pipetting
Example: Fill 250 µl and dispense 45 µl in the first column of the
microtiter plate. Reduce each subsequent column by 5 µl for a
total of 8 columns. Discard the remainder.
[Fill]
s
t
Vol
(250) [Enter]
[Disp]
s
t
Vol
(45) [Enter]
[Disp]
s
t
Vol
(40) [Enter]
[Disp]
s
t
Vol
(35) [Enter]
[Disp]
s
t
Vol
(30) [Enter]
[Disp]
s
t
Vol
(25) [Enter]
[Disp]
s
t
Vol
(20) [Enter]
[Disp]
s
t
Vol
(15) [Enter]
[Disp]
s
t
Vol
(10) [Enter]
[Purge] [Enter] [Enter]
A purge step is entered as the last step in the program to limit the
dispense step to eight columns; otherwise, the pipettor would
dispense 10 µl until it was empty.
F.1.3 Serial Dilutions
Example: Transfer 50 µl to the first column of a plate, mix 100 µl.
Follow this procedure for exactly 4 columns.
[Fill]
s
t
Vol
(50) [Enter]
[Mix]
s
t
Vol
(100) [Enter]
[Mix] [Enter]
[Mix] [Enter]
[Mix] [Enter]
[Purge] [Enter] [Enter]
This program requires a specified number of steps; therefore, each
mix step is entered, and the program is ended with a purge step.
Since the subsequent mix steps use the same volume, the volume
does not need to be entered each time.
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