Merck Millipore ReadyStream - Sanitization of the internal fluidic path

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The following reagent is recommended for internal
cleaning:
Reagent Supplier
Quaternary ammonium: Benzalkonium chloride (between
0.25–0.5%) Poly(hexamethylene biguanide) hydrochloride
(between 0.1–0.25%)
Contact
our local
technical
service
Quaternary ammonium : Benzalkonium chloride
(between 0.05%-0.1%), tetrasodium ethylene diamine
tetraacetate (between 50-500ppm), N-(3-aminopropyl)-
N-dodecylpropane-1,3-diamine (between 50-150ppm)
Isopropanol and ethanol should not be used
for internal ow path cleaning.
For sanitization of the uidic path, use the specic
sanitization screw cap with tubing provided with the
instrument. This screw cap can be used on any GL45
bottle of 1 L or 2 L (not provided).
Do not connect to bottle smaller than 1 L.
For the sanitization, the decontamination uid is
recirculated three times inside the instrument for a
total contact time of 15 min. The instrument is then
rinsed, either by reconnecting the instrument to the
water source and ushing 2 times 2.5 L of water
with the outlet sanitization tubing connected to the
instrument outlet port, or by recirculating 10 times
0.5 L of water using the sanitization kit.
To start the sanitization process, turn ON the
instrument, and scan a specic sanitization data matrix
instead of the lter label, then follow the instructions
on the display.
Example 1: (see complete description below)
Three recirculations of 250 mL of sanitizing solution,
each with a 5 minute contact time, followed by 10
rinsing cycles, each using 500 mL of fresh deionized
water.
Example 2: (alternative solution with larger
rinsing container)
Three recirculations of 250 mL of sanitizing solution,
each with a 5 minute contact time, followed by 2
rinsing cycles, each using 2500 mL of fresh deionized
water.
Both “Priority” LEDs will blink green to indicate that the
instrument is in the sanitization mode and will continue to
blink during this process.
Disconnect any lter/media bag from the instrument,
disconnect the water inlet tubing and connect the
sanitization kit to the instrument, with the straight
connector on the water inlet port of the instrument (Figure
2, L1), and the male elbow connector on the instrument
outlet port (Figure 1, F1). Press [+] to conrm that the
tubings are in place.
The user is then prompted to add the sanitizer to the
GL45 bottle (not provided with the sanitization kit).
Screw the sanitization kit cap on the bottle. Press [+]
to conrm that the system is ready to start sanitization.
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