6-3
Figure 6-1 Logit-Log 4P calibration curve
R
C
C1 C2 C3 C4
Logistic-Log 5P
Calibration formula:
)]ln(exp[1
1
0
cCCba
KRR
++−+
+=
This calibration method adopts five parameters:
0
R ,
, a , b and c .
This calibration method requires at least five calibrators. The concentration (or
activity) of calibrator 1 is 0, and the corresponding
is equal to
0.
The
applications of the calibration method are the same with that of Logit-Log 4P, but this
method has a higher fitting.
Exponential 5P
Calibration formula: ])(ln)(lnlnexp[
32
0
CcCbCaKRR +++=
This calibration method adopts five parameters:
0
R ,
, a , b and c .
This calibration method requires at least five calibrators. The concentration (or
activity) of calibrator 1 is 0, and the corresponding
is equal to
0
R
0.
This
calibration method is applied to the calibration curve that the response increases
sharply when the concentration reaches a specific value. See Figure 6-2.
Figure 6-2 Exponential 5p calibration curve
R
C
C1 C2 C3 C4 C5
Polynomial 5P
Calibration formula:
3
0
2
00
)
100
()
100
()
100
(ln
RR
d
RR
c
RR
baC
−
+
−
+
−
+=