6-3
Figure 6-1 Logit-Log 4P calibration curve 
R
C
C1 C2 C3 C4
 
Logistic-Log 5P 
Calibration formula: 
)]ln(exp[1
1
0
cCCba
KRR
++−+
+=  
This calibration method adopts five parameters: 
0
R , 
,  a ,  b  and  c . 
This calibration method requires at least five calibrators. The concentration (or 
activity) of calibrator 1 is 0, and the corresponding 
 is equal to 
0.
The 
applications of the calibration method are the same with that of Logit-Log 4P, but this 
method has a higher fitting. 
Exponential 5P 
Calibration formula:  ])(ln)(lnlnexp[
32
0
CcCbCaKRR +++=  
This calibration method adopts five parameters: 
0
R , 
,  a ,  b  and  c . 
This calibration method requires at least five calibrators. The concentration (or 
activity) of calibrator 1 is 0, and the corresponding 
 is equal to 
0
R
0.
This 
calibration method is applied to the calibration curve that the response increases 
sharply when the concentration reaches a specific value. See Figure 6-2. 
Figure 6-2 Exponential 5p calibration curve 
R
C
C1 C2 C3 C4 C5
 
Polynomial 5P 
Calibration formula: 
3
0
2
00
)
100
()
100
()
100
(ln
RR
d
RR
c
RR
baC
−
+
−
+
−
+=