Molecular Devices ImageXpress Pico User Manual

ImageXpress Pico Automated Cell Imaging System Installation Guide
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Objective Lenses
The ImageXpress Pico system can be configured with high-quality Leica Fluotar objectives.
Five objectives are currently available.
You can identify the magnification of an objective by the color band:
Objective Magnification Color Band
4x Red
10x Yellow
20x Green
40x Light Blue
63x Dark Blue
The 40x objective and 63x objective have application-optimized correction collars to
compensate for well bottom thickness or coverslip thickness. The collars have a range of
0mm to 2mm correction. Changing this setting adjusts the distances between components
inside the objective barrel. Image quality and resolution are very dependent on properly
setting these collars.
The settings to be used depend on the well bottom thickness of the plate or the coverslip
thickness on the slide on which the specimen is mounted. In general, set the correction collar
for the physical thickness of the plate or slide that you are imaging. The physical thickness can
be determined by the plate specifications from the plate manufacturer.
See Objectives on page 71 for details.

Molecular Devices ImageXpress Pico Specifications

General

Brand	Molecular Devices
Model	ImageXpress Pico
Category	Laboratory Equipment
Language	English