Chapter 8 Troubleshooting
2 Image Output
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Symptom Corrective action
The contrast is low.
• Set the tone appropriately using the application software.
=> To make settings, see the instructions for the application software.
• Set the black level to the appropriate value using the application
software.
=> To make settings, see the instructions for the application software.
• Adjust the size of the field diaphragm so that it circumscribes the
viewfield.
• Adjust the size of the aperture diaphragm to approximately 70% to
80% of the numerical aperture of the objective.
• Use an appropriate filter.
When using a phase contrast microscope or an interference
microscope (two luminous fluxes or multiple luminous fluxes), insert
the specified filter. With a black and white image, contrast is improved
by inserting a green interference filter (GIF). Contrast in color images
is enhanced by inserting a filter having a complementary color to that
of the specimen.
• Adjust the ambient light around the microscope.
Make the room darker, or place caps on the binocular eyepiece, for
example.
• Check the contrast of the specimen itself.
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