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Nikon Eclipse 50i - Automatic Adjustment after Magnification Change (Only for a 55 I When a Cytodiagnostic Unit Is Mounted); Adjustment with the ND Filter for the Fluorescence Attachment; Transmitted Image in Fluorescence Observation

Nikon Eclipse 50i
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Chapter 3 Individual Operations
3.2 Brightness Adjustment
38
3.2.4
Automatic adjustment after magnification change
(only for a 55i when a cytodiagnostic unit is mounted)
If the cytodiagnostic unit is mounted to a 55i,
pressing the hand switch to change magnification
will also activate brightness adjustment.
However, the brightness control knob must not be
at the maximum setting, and the preset switch
must not be in the depressed position for this
automatic adjustment function to activate.
3.2.5
Adjustment with the ND filter for the fluorescence attachment
Pushing in the ND filter attach/detach lever moves
the ND filter into the optical path and darkens the
fluorescent image.
ND filters are used to adjust light intensity. Higher
filter numbers correspond to lower transmission
rates (i.e., darker images). ND filters do not affect
color balance. (The table on P.53 shows the
brightness levels achieved by different
combinations of the three filters.)
ND2: Reduces light intensity by 1/2.
ND4: Reduces light intensity to 1/8 previous levels.
ND16: Reduces light intensity to 1/16 previous levels.
3.2.6
Transmitted image in fluorescence observation
For fluorescence observations, turn off the microscope power switch to cancel the transmitted
image.
Bright ambient lights will make it more difficult to view the image. We recommend keeping the
room dark during fluorescence observations.
Hand switch
ND filter

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