Chapter 3 Individual Operations
3.17 Selecting Fluorescent Filters
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3.17
Selecting Fluorescent Filters
A filter cube is comprised of the following three
optical components: an excitation filter (EX filter),
a barrier filter (BA filter), and a dichroic mirror
(DM). Select a combination of filter cubes
appropriate for the specimen characteristics,
fluorescent pigment, and the purpose intended.
Keep in mind the following:
• Different combinations of excitation and barrier
filters may be selected for the same excitation
method.
• Other types of excitation filters, barrier filters,
and dichroic mirrors can be purchased
separately.
• Excitation filters are exposed to strong light
during operations and tend to age rapidly.
Replace the excitation filter at frequent
intervals.
When using the UV2A or UV2B, be sure to remove the screw from the excitation
filter frame and the spacer from inside the excitation filter. These filters cannot
be used until the spacer ring has been removed.
(Refer to 3.7.4.)
Excitation
filter
Barrier filter
Dichroic mirror
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