Nikon Eclipse 50i - Replacing Excitation and Barrier Filters; Filter Cube Internal Spacers

Chapter 3 Individual Operations
3.17 Selecting Fluorescent Filters
57
3
3.17.3
Replacing excitation and barrier filters
Excitation and barrier filters can be removed from
the fluorescent cube and replaced. (Dichroic
mirrors cannot be dismounted from the fluorescent
cube.) Excitation filters are screw-in filters.
Barrier filters are slide-in filters. Align the
projection on the barrier filter with the groove on
the fluorescent cube and turn clockwise
approximately 30 degrees to secure in place.
3.17.4
Filter cube internal spacers
The filter cubes listed below cannot be inserted
directly into the fluorescence attachment. Instead,
as described below, an internal spacer must first
be removed or reversed.
(1) Place the filter cube on the work surface with
the excitation filter facing up.
(2) Unscrew the ring retaining the excitation
filter.
(Be careful to avoid dropping the filter.)
(3) Remove the spacer inside the ring.
(4) Remove or reverse the spacer as appropriate
for the particular filter cube type. The actions
suitable for various filter cubes are given
below.
(5) Reattach the ring.
- Filter cubes requiring spacer removal
• UV-2A
• UV-2B
- Filter cubes requiring spacer reversal
• DAPI
• FITC
• GFP-L
• GFP-B
• TRITC
• Tx-Red
Barrier filter
Excitation
filter
T
urn about 30° to secure in
p
lace.
S
p
acer
Filter cube
Rin
g