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Nikon Eclipse 50i - Fluorescence Microscopy Guide

Nikon Eclipse 50i
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Chapter 2 Microscopy
2.3 Microscopy with Fluorescence Attachment Mounted
29
2
2.3
Microscopy with Fluorescence Attachment
Mounted
Before microscopy
Check the cumulative operating hours of the mercury lamp. Replace the lamp if
its cumulative operating hours exceed the average service life.
Use non-fluorescent slide glass.
Use non-fluorescent immersion oil.
To keep the specimen color from fading, keep the shutter closed when not
performing microscopy.
1
Perform steps 1 through 10 in “1 Bright-Field Microscopy.”
2
Turn off the microscope power
switch.
3
Close the shutter and block the
light emitted by the mercury lamp.
P.51
4
Insert the excitation filter cube to
be viewed into the optical path.
P.52
Close
the shutter.
Select a cube using the filter
cube switching knob.
Press the
p
ower switch
to the “” position.

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