Nikon Eclipse 50i - Page 32

Chapter 2 Microscopy
2.3 Microscopy with Fluorescence Attachment Mounted
30
5
Fully open the field diaphragm of
the fluorescence attachment.
⇒
⇒⇒
⇒ P.51
6
Turn on the mercury lamp, then
open the shutter and center the
lamp. (Refer to the operating
manual for the light source.)
7
Set the 10x objective into the
optical path.
8
Set a specimen and move the
portion to be viewed into the
optical path.
⇒
⇒⇒
⇒ P.42
9
Focus on the specimen.
⇒
⇒⇒
⇒ P.40
Full
y
o
p
en the field dia
p
hra
g
m.
Select the
10x objective.
Set a specimen
and secure in
place using the
specimen
holder.
Move the portion to be viewed into the
optical path using the XY stage knobs.
Focus on the specimen using
the coarse and fine focus knobs.